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TMB-D Pharmaceutical Intermediate 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate

Place of Origin China
Brand Name N/A
Certification COA
Model Number SFC310
Minimum Order Quantity 1kg
Price USD1,500-USD2,150/kg
Packaging Details 20kg or 25kg/drum
Delivery Time 5-8 working days
Payment Terms T/T, L/C, D/A, D/P, Western Union
Supply Ability 5000kg/month

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Product Details
Purity ≥98.0% MF C16H20N2.2ClH
MW 313.27 CAS 207738-08-7
Appearance White Powder Application Pharmaceutical Intermediate
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TMB-D pharmaceutical intermediate


207738-08-7 pharmaceutical intermediate


CAS 207738-08-7

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Product Description

TMB-2HCL-2HO CAS 207738-08-7 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate




CAS 207738-08-7
Purity ≥98.0%
MF C16H20N2.2ClH
MW 313.27
Appearance White powder
Application pharmaceutical intermediate





TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.



1. Quick lead time

2. Low MOQ

3. Quality assured with COA

4. Full experience in shipping

5. Free consulting service

6. custom service welcome




Q1: What is your regular lead time for sample and official order?

A1: Sample lead time 5-7 days. Official order lead time 14-21 days.


Q2: What is your MOQ?

A2: MOQ usually is 1kg.


Q3: How you control product quality?

A3: From raw material inspection, dupro inspection to final product inspection are executed strictly.




Item Photo

TMB-D Pharmaceutical Intermediate 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate 0

TMB-D Pharmaceutical Intermediate 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate 1